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t denticola atcc 35405 wild type strain  (ATCC)


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    Structured Review

    ATCC t denticola atcc 35405 wild type strain
    T Denticola Atcc 35405 Wild Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 757 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 757 article reviews
    t denticola atcc 35405 wild type strain - by Bioz Stars, 2026-10
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    Related Articles

    Mutagenesis:

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex
    Article Snippet: .. T. denticola strains are as in Fig. 2: 35405 (ATCC 35405; parent strain), CF417 (prcB-6 His polar mutation), CF499 (prcB-6 His nonpolar mutation), CF522 ( prcB), and P0760 (erm insertion at 5 end of prcB). on A pril 11, 2015 by U niversity of W estern O ntario http://jb.asm .org/ D ow nloaded from activity, we constructed CF522, a prcB mutant in which the putative protease locus promoter region is intact (Fig. 2). ..

    Article Title: Disruption of a Type II Endonuclease (TDE0911) Enables Treponema denticola ATCC 35405 To Accept an Unmethylated Shuttle Vector
    Article Snippet: .. Briefly, three T. denticola strains (ATCC 35405, ATCC 33520, and the TDE0911 mutant) were grown to early logarithmic phase (optical density at 600 nm of 0.3 to 0.4), and the cells were enumerated using Petroff-Hausser counting chambers. ..

    Article Title: Disruption of a Type II Endonuclease (TDE0911) Enables Treponema denticola ATCC 35405 To Accept an Unmethylated Shuttle Vector
    Article Snippet: .. Briefly, three T. denticola strains (ATCC 35405, ATCC 33520, and the TDE0911 mutant) were grown to early logarithmic phase (optical density at 600 nm of 0.3 to 0.4), and the cells were enumerated using Petroff-Hausser counting chambers. ..

    Article Title: A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405
    Article Snippet: Images were analyzed using Nikon EZ-C1 software. .. T. denticola strains, including ATCC 33520 and ATCC 35405 as well as isogenic 35405 mutant 35405-ΔTDE0911 ( 3 ), were grown as previously described ( 9 ) under anoxic conditions in NOS broth or semisolid medium containing 0.8% Noble agar, supplemented with erythromycin (Em; 40 μg ml −1 ) and chloramphenicol (Cm; 10 μg ml −1 ) as appropriate. ..

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex
    Article Snippet: .. T. denticola strains are as in Fig. : 35405 (ATCC 35405; parent strain), CF417 ( prcB -6×His polar mutation), CF499 ( prcB -6×His nonpolar mutation), CF522 (Δ prcB ), and P0760 ( erm insertion at 5′ end of prcB ). ..

    Activity Assay:

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex
    Article Snippet: .. T. denticola strains are as in Fig. 2: 35405 (ATCC 35405; parent strain), CF417 (prcB-6 His polar mutation), CF499 (prcB-6 His nonpolar mutation), CF522 ( prcB), and P0760 (erm insertion at 5 end of prcB). on A pril 11, 2015 by U niversity of W estern O ntario http://jb.asm .org/ D ow nloaded from activity, we constructed CF522, a prcB mutant in which the putative protease locus promoter region is intact (Fig. 2). ..

    Construct:

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex
    Article Snippet: .. T. denticola strains are as in Fig. 2: 35405 (ATCC 35405; parent strain), CF417 (prcB-6 His polar mutation), CF499 (prcB-6 His nonpolar mutation), CF522 ( prcB), and P0760 (erm insertion at 5 end of prcB). on A pril 11, 2015 by U niversity of W estern O ntario http://jb.asm .org/ D ow nloaded from activity, we constructed CF522, a prcB mutant in which the putative protease locus promoter region is intact (Fig. 2). ..

    Plasmid Preparation:

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.



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    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent <t>ATCC</t> <t>35405</t> and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.
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    ATCC t denticola strain atcc 35405 coaggregate
    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent <t>ATCC</t> <t>35405</t> and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.
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    ATCC t denticola genomic dna
    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent <t>ATCC</t> <t>35405</t> and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.
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    Image Search Results


    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent ATCC 35405 and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.

    Journal: Molecular oral microbiology

    Article Title: Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids

    doi: 10.1111/omi.70016

    Figure Lengend Snippet: Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent ATCC 35405 and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.

    Article Snippet: To further characterize shuttle plasmid-mediated complementation in T. denticola ATCC 35405, we used several different promoter/gene of interest combinations in complementation studies of T. denticola ATCC 35405 mutant strains ( and ).

    Techniques: Expressing, Mutagenesis, Transformation Assay, Plasmid Preparation, Control, Derivative Assay, Western Blot, Electrophoresis